General Cell Based Assays
Eurofins Discoverx PathHunter eXpress DDR1 Functional Assay, 10-Plate Kit. (93-0894E3CP16L)
The PathHunter eXpress DDR1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated DDR1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged DDR1 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM4 Total Internalization Assay, 2pl Kit. (93-0902E3CP0M)
The PathHunter CHRM4 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM4 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CHRM4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CHRM4 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL21R/IL2RG Dimerization Assay, 2pl Kit. (93-1035E3CP0M)
The PathHunter IL21R/IL2RG Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-21) induced dimerization of IL21R/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL21RG target protein fused to PK alongside IL2R target protein fused to EA. Upon ligand-induced dimerization of IL21R/IL2RG, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Apexbio Technology LLC 2-NBDG Glucose Uptake Assay Kit(Synonyms: 2-NBDG Glucose Assay Kit, Fluorescent Glucose Uptake Kit), 500T.
2-NBDG Glucose Uptake Assay Kit utilizes the fluorescently labeled glucose analog 2-NBDG (2-N-(7-nitrobenz-2-oxa-1 3-diazol-4-yl)amino-2-deoxyglucose) to quantify glucose uptake in cells 2-NBDG enters cells via glucose transporters (GLUTs) and undergoes phosphorylation at the C-6 position producing 2-NBDG-6-phosphate thus becoming intracellularly trapped This property enables precise measurement of cellular glucose uptake at the single-cell level through fluorescence analysis Relevant in metabolic pathway research and conditions including cancer diabetes and obesity this assay provides a rapid sensitive and non-radioactive approach to monitoring glucose metabolism
Aat Bioquest Amplite™ Fluorimetric NAD Assay Kit *Blue Fluorescence*
Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.
Aat Bioquest Amplite™ Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation.
ACROBiosystems Human TL1A-DR3 Inhibition Kit (TR-FRET), 100 tests
Human TL1A-DR3 Inhibition Kit (TR-FRET) is a homogeneous, no-wash competition assay for rapid screening of inhibitors that block TL1A binding to DR3. The assay uses time-resolved fluorescence resonance energy transfer for sensitive, high-throughput detection and produces results within 0.5-1 hour.
- Homogeneous competition TR-FRET assay for inhibitor screening.
- Available in 100-test and 500-test kit sizes; larger quantities by inquiry.
- Includes Europium-chelate labeled TL1A, FA-labeled DR3, control antibody, and TR-FRET buffers.
- Assay time 0.5-1 hour; typical completion in 1 hour.
- Store unopened kit at 2-8 °C; opened kit stable for 30 days.
- Validated with antibody inhibitors; demonstrated IC50 values in the low nanomolar range.
Eurofins Discoverx PathHunter eXpress GCGR CHO-K1 β-Arrestin-1 GPCR Assay, 10-Plate Kit. (93-0979E2CP2L)
The PathHunter GCGR (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. glucagon) based activation of GCGR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GCGR and EA-tagged β-Arrestin-1. Activation of GCGR stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY4 Activated GPCR Internalization Assay, 2pl Kit. (93-0741E3CP0M)
The PathHunter P2RY4 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY4 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY4. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY4-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mEDNRA CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0685E2MCP0M)
The PathHunter mEDNRA eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-1) based activation of mEDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRA and EA-tagged β-Arrestin-2. Activation of mEDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTHR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0770E3CP0L)
The PathHunter PTHR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTHR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged PTHR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting PTHR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGFR Total Internalization Assay, 10pl Kit. (93-0872E3CP0L)
The PathHunter PTGFR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGFR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGFR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGFR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cyno CXCR2 HEK 293 β-Arrestin GPCR Assay, 2pl Kit. (93-0981E1CCP0M)
The PathHunter cyno CXCR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of cyno CXCR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno CXCR2 and EA-tagged β-Arrestin-2. Activation of cyno CXCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TYK2 Activity Assay, 10-Plate Kit. (93-0875E3CP16L)
The PathHunter eXpress TYK2 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the TYK kinase domain. The cell line is engineered to express the catalytic domain from TYK2 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the TYK2 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPBWR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0818E2CP2M)
The PathHunter NPBWR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptides B/W) based activation of NPBWR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPBWR1 and EA-tagged β-Arrestin-2. Activation of NPBWR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.